Gavi´s Site

La cristalografía de proteínas, ¿por qué y para qué?

Chemical Science June 2020 issue

GNCA-STanalog

Enhancing a de novo enzyme activity by computationally-focused ultra-low-throughput screening

Chemical Science, (2020) 11, 6134-6148. Risso, V.A., Romero-Rivera, A., Gutierrez-Rus, L.I., Ortega-Muñoz, M., Santoyo-Gonzalez, F., Gavira, J.A., Sanchez-Ruiz, J.M. & Kamerlin, S.C.L.,
doi: 10.1039/D0SC01935F

HOT ARTICLE

Pick of the Week

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HUMAN FRONTIER Science Program »

#INFLUYENTESdeVERDAD2019

Silica-gel

CG&D August 2019 issue

Fmoc-gel

Enhanced Stability against Radiation Damage of Lysozyme Crystals Grown in Fmoc-CF Hydrogels

CG&D , (2019) 19, 4229-33. Contreras-Montoya, R., Castellvi, A., Escolano-Casado, G., Juanhuix, J., Conejero-Muriel, M., Lopez-Lopez, M.T., Cuerva, J.M., Alvarez de Cienfuegos, L. and Gavira, J.A.,
doi: 10.1021/acs.cgd.9b00131

ActaD Special Issue on Neutron scattering

Silica-gel

Seeding from silica-reinforced lysozyme crystals for neutron crystallography

Acta Cryst. D74, (2018) 1200-7. Gavira, J.A., Conejero-Muriel, M. and Delgado-López, J.M.,
doi: 10.1107/S2059798318016054

HYDROgels

Peptide hydrogel

Article »

Protein crystallization in short-peptide supramolecular hydrogels: a versatile strategy towards biotechnological composite materials

CrystEngComm, (2015) 17, 8072-78. Conejero-Muriel, Mayte, Contreras-Montoya, Rafael, Díaz-Mochón, Juan J., Álvarez de Cienfuegos, Luis and Gavira, José A.
doi: 10.1039/c5ce00850f

IDEAL: "Patentan un nuevo hidrogel que abre las puertas al desarrollo de fármacos más efectivos...." More »

Peptide hydrogel

Article »

Influence of the chirality of short peptide supramolecular hydrogels in protein crystallogenesis

Chem Commun, (2015) 51, 3862-65. Conejero-Muriel, M., Gavira, J. A., Pineda-Molina, E., Belsom, A., Bradley, M., Moral, M., Duran, J. D., Luque Gonzalez, A., Diaz-Mochon, J. J., Contreras-Montoya, R., Martinez-Peragon, A., Cuerva, J. M. and Alvarez de Cienfuegos, L. doi: 10.1039/c4cc09024a

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ASTROBIOLOGY

Back from the Dead: Scientists "Resurrect" Ancient Proteins to Learn about Primordial Life on Earth. Geological evidence tells us that ancient Earth probably looked and felt very different from the planet we all recognize today. Billions of years ago, our world was a comparatively harsh place.... More »

Ancestral Tactamase

Article »

Phenotypic comparisons of consensus variants versus laboratory resurrections of Precambrian proteins

Proteins, (2014) 82, 887-96. Risso, V. A., Gavira, J. A., Gaucher, E. A. and Sanchez-Ruiz, J. M. doi: 10.1002/prot.24575

Xfel-HUB - Lecture March 17 2022 -

IYCr crystallization series

Introduction to protein crystallization

A. McPherson and J. A. Gavira

Synopsis: The gateway to X-ray crystallography is the growth of macromolecular crystals. An attempt is made here to acquaint investigators with the principles and practice of protein crystallization, some recent innovations, and to touch upon some of the remaining problems.

Article »

Conservation of Protein Structure over Four Billion Years.

Structure, (2013) 21, (9), 1690-1697. Ingles-Prieto, A.; Ibarra-Molero, B.; Delgado-Delgado, A.; Perez-Jimenez, R.; Fernandez, J. M.; Gaucher, E. A.; Sanchez-Ruiz, J. M.; Gavira, J. A. doi: 10.1016/j.str.2013.06.020

ACS News: Resurrection of 3-billion-year-old antibiotic-resistance proteins

Scientists are reporting "laboratory resurrections" of several 2-3-billion-year-old proteins that are ancient ancestors of the enzymes that enable today's antibiotic-resistant bacteria to shrug off huge doses of penicillins, cephalosporins and other modern drugs..... More »

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Worldpress »

Alactamase

Article »

Hyperstability and Substrate Promiscuity in Laboratory Resurrections of Precambrian b-Lactamases.

J Am Chem Soc, (2013) 135, (8), 2899-902. Risso, V. A.; Gavira, J. A.; Mejia-Carmona, D. F.; Gaucher, E. A.; Sanchez-Ruiz, J. M. doi: 10.1021/ja311630a

BBC: Resurrected protein's clue to origins of life

New reconstructions of ancient proteins have provided clues to the habitat and origins of life on Earth. The resurrected protein is thought to have existed almost four billion years ago in single-celled organisms linked to the earliest ancestor of all life..... More »

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AAAS Eurekalert » Livescience » SINC » El Mundo »

Ancestral Thioredoxins

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ESRF Scientists "resurrect" ancient proteins to provide clues about molecular innovation

A growing body of research on the use of ancestral protein resurrection has been published in recent years to address a variety of issues on protein evolution and protein engineering. In a study published in Nature Communications, an International team of scientists has demonstrated the high potential of this evolutionary approach to endow new activities into ancient enzymes...... More »